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重組蛋白表達系列一
哺乳動物細胞表達系統(tǒng)
展示案例：HEK293F Expression System
（1）Protein Name: protein L（一種scfv）
該蛋白不易表達，采用哺乳動物細胞表達系統(tǒng)表達活性最佳，其他表達系統(tǒng)表達活性低。
（2）Expression vector: pATX2
（3）Expression host: HEK293F
總結(jié)：蛋白L（scfv）采用普健高表達pATX系列載體配合公司優(yōu)化高密度哺乳表達系統(tǒng)Xten293，成功表達，表達量高達31.2mg/L，純度>90%，經(jīng)檢驗，其具有較高活性。
以圖為鑒
Test Report
01
Expression test
MW. Molecular weight marker. Ø. Non-transfected (negative control).
The protein L expressed secretorily in HEK293F cells.
02
Purification Test
MW. Molecular weight marker. IN. Input. FT. Flow through. W. Washes. E. Eluted fractions.
A. The purification test result. B. Final QC gel.
L-pATX2 was transfected with 80ml HEK293F cells. The culture medium and cells which were collected on the sixth day post transfection did purification test.
03
Fermentation and Purification
1L scale up purification
MW. Molecular weight marker. IN. Input. FT. Flow through. W. Washes. E. Eluted fractions.
A. The purification test result. B. Final QC gel.
普健生物哺乳動物細胞表達服務(wù)
瞬時轉(zhuǎn)染表達
重組抗體表達
穩(wěn)定細胞株構(gòu)建
大規(guī)模生產(chǎn)服務(wù)
